Summary:

 

Purpose

 

Our Quick Step Human 3β-Hydroxysteroid Dehydrogenase type 2 (HSD3B2)elisa Kit (Enzyme activity) is to assay HSD3B2 levels in Human serum, plasma, culture media or any biological fluid.

 

Principle

 

This ELISA kit uses Sandwich-ELISA as the method. The Microelisa stripplate provided in this kit has been pre-coated with an antibody specific to HSD3B2. Standards or samples are added to the appropriate Microelisa stripplate wells and combined to the specific antibody. Then a Horseradish Peroxidase (HRP)- conjugated antibody specific for HSD3B2 is added to each Microelisa stripplate well and incubated. Free components are washed away. The TMB substrate solution is added to each well. Only those wells that contain HSD3B2 and HRP conjugated HSD3B2 antibody will appear blue in color and then turn yellow after the addition of the stop solution. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of HSD3B2. You can calculate the concentration of HSD3B2 in the samples by comparing the OD of the samples to the standard curve.

 

Typical Data

 

The standard curve of QSD3878Hu is provided for demonstration only. A standard curve should be generated for each set of samples assayed.

 

 

 

 

Standard	Concentration	OD Value	Average OD Value
Blank Well	0U/L	0.043	0.0405
		0.038
S1	90U/L	1.954	1.9615
		1.969
S2	60U/L	1.318	1.3305
		1.343
S3	30U/L	0.683	0.6910
		0.699
S4	15U/L	0.391	0.3865
		0.382
S5	7.5U/L	0.194	0.1910
		0.188

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