Summary:

 

Purpose

 

Our Quick Step Mouse lymphocyte function associated antigen 3,LFA-3 ELISA Kit is to assay LFA-3 levels in Mouse serum, plasma, culture media or any biological fluid.

 

Principle

 

This ELISA kit uses Sandwich-ELISA as the method. The Microelisa stripplate provided in this kit has been pre-coated with an antibody specific to LFA-3. Standards or samples are added to the appropriate Microelisa stripplate wells and combined to the specific antibody. Then a Horseradish Peroxidase (HRP)- conjugated antibody specific for LFA-3 is added to each Microelisa stripplate well and incubated. Free components are washed away. The TMB substrate solution is added to each well. Only those wells that contain LFA-3 and HRP conjugated LFA-3 antibody will appear blue in color and then turn yellow after the addition of the stop solution. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of LFA-3. You can calculate the concentration of LFA-3 in the samples by comparing the OD of the samples to the standard curve.

 

Typical Data

 

The standard curve of QS0358Mo is provided for demonstration only. A standard curve should be generated for each set of samples assayed.

 

 

 

 

Standard	Concentration	OD Value	Average OD Value
Blank Well	0pg/ml	0.043	0.0405
		0.038
S1	90pg/ml	1.954	1.9615
		1.969
S2	60pg/ml	1.318	1.3305
		1.343
S3	30pg/ml	0.683	0.6910
		0.699
S4	15pg/ml	0.391	0.3865
		0.382
S5	7.5pg/ml	0.194	0.1910
		0.188

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