Summary:

 

Purpose

 

Our Quick Step Human S-Equol ELISA Kit is to assay  S-Equol levels in Human serum, plasma, culture media or any biological fluid.

 

Principle

 

This ELISA kit uses Sandwich-ELISA as the method. The Microelisa stripplate provided in this kit has been pre-coated with an antibody specific to  S-Equol. Standards or samples are added to the appropriate Microelisa stripplate wells and combined to the specific antibody. Then a Horseradish Peroxidase (HRP)- conjugated antibody specific for  S-Equol is added to each Microelisa stripplate well and incubated. Free components are washed away. The TMB substrate solution is added to each well. Only those wells that contain  S-Equol and HRP conjugated  S-Equol antibody will appear blue in color and then turn yellow after the addition of the stop solution. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of  S-Equol. You can calculate the concentration of  S-Equol in the samples by comparing the OD of the samples to the standard curve.

 

Typical Data

 

The standard curve of QSD4188Hu is provided for demonstration only. A standard curve should be generated for each set of samples assayed.

 

 

 

 

Standard	Concentration	OD Value	Average OD Value
Blank Well	0ng/L	0.048	0.0505
		0.053
S1	180ng/L	1.941	1.9370
		1.933
S2	120ng/L	1.317	1.3255
		1.334
S3	60ng/L	0.709	0.7200
		0.731
S4	30ng/L	0.373	0.3645
		0.356
S5	15ng/L	0.187	0.1900
		0.193

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