Summary:

 

Purpose

 

Our Quick Step Mouse Mesencephalic Astrocyte-Derived Neurotrophic Factor (MANF)ELISA Kit is to assay MANF levels in Mouse serum, plasma, culture media or any biological fluid.

 

Principle

 

This ELISA kit uses Sandwich-ELISA as the method. The Microelisa stripplate provided in this kit has been pre-coated with an antibody specific to MANF. Standards or samples are added to the appropriate Microelisa stripplate wells and combined to the specific antibody. Then a Horseradish Peroxidase (HRP)- conjugated antibody specific for MANF is added to each Microelisa stripplate well and incubated. Free components are washed away. The TMB substrate solution is added to each well. Only those wells that contain MANF and HRP conjugated MANF antibody will appear blue in color and then turn yellow after the addition of the stop solution. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of MANF. You can calculate the concentration of MANF in the samples by comparing the OD of the samples to the standard curve.

 

Typical Data

 

The standard curve of QS1020Mo is provided for demonstration only. A standard curve should be generated for each set of samples assayed.

 

 

 

 

Standard	Concentration	OD Value	Average OD Value
Blank Well	0pg/ml	0.048	0.0505
		0.053
S1	180pg/ml	1.941	1.9370
		1.933
S2	120pg/ml	1.317	1.3255
		1.334
S3	60pg/ml	0.709	0.7200
		0.731
S4	30pg/ml	0.373	0.3645
		0.356
S5	15pg/ml	0.187	0.1900
		0.193

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