Summary:

 

Purpose

 

Our Quick Step Mouse F2-isoprostane (F2-iso)elisa Kit is to assay F2-iso levels in Mouse serum, plasma, culture media or any biological fluid.

 

Principle

 

This ELISA kit uses Sandwich-ELISA as the method. The Microelisa stripplate provided in this kit has been pre-coated with an antibody specific to F2-iso. Standards or samples are added to the appropriate Microelisa stripplate wells and combined to the specific antibody. Then a Horseradish Peroxidase (HRP)- conjugated antibody specific for F2-iso is added to each Microelisa stripplate well and incubated. Free components are washed away. The TMB substrate solution is added to each well. Only those wells that contain F2-iso and HRP conjugated F2-iso antibody will appear blue in color and then turn yellow after the addition of the stop solution. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of F2-iso. You can calculate the concentration of F2-iso in the samples by comparing the OD of the samples to the standard curve.

 

Typical Data

 

The standard curve of QSD1013Mo is provided for demonstration only. A standard curve should be generated for each set of samples assayed.

 

 

 

 

Standard	Concentration	OD Value	Average OD Value
Blank Well	0ng/L	0.057	0.0550
		0.053
S1	240ng/L	1.968	1.9635
		1.959
S2	160ng/L	1.392	1.3385
		1.285
S3	80ng/L	0.710	0.7190
		0.728
S4	40ng/L	0.376	0.3855
		0.395
S5	20ng/L	0.205	0.1990
		0.193

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