Summary:

 

Purpose

 

Our Quick Step Human anti acetylated ornithin antibodies (AcOrn)elisa Kit is to assay AcOrn levels in Human serum, plasma, culture media or any biological fluid.

 

Principle

 

This ELISA kit uses Sandwich-ELISA as the method. The Microelisa stripplate provided in this kit has been pre-coated with an antigen specific to AcOrn. Standards or samples are added to the appropriate Microelisa stripplate wells and combined to the specific antigen. Then a Horseradish Peroxidase (HRP)- conjugated antigen specific for AcOrn is added to each Microelisa stripplate well and incubated. Free components are washed away. The TMB substrate solution is added to each well. Only those wells that contain AcOrn and HRP conjugated AcOrn antigen will appear blue in color and then turn yellow after the addition of the stop solution. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of AcOrn. You can calculate the concentration of AcOrn in the samples by comparing the OD of the samples to the standard curve.

 

Typical Data

 

The standard curve of QS3800Hu is provided for demonstration only. A standard curve should be generated for each set of samples assayed.

 

 

 

 

Standard	Concentration	OD Value	Average OD Value
Blank Well	0ng/ml	0.057	0.0550
		0.053
S1	240ng/ml	1.968	1.9635
		1.959
S2	160ng/ml	1.392	1.3385
		1.285
S3	80ng/ml	0.710	0.7190
		0.728
S4	40ng/ml	0.376	0.3855
		0.395
S5	20ng/ml	0.205	0.1990
		0.193

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