Summary:

 

Purpose

 

Our Quick Step Mouse Phosphatidylinositol 3-kinase regulatory subunit gamma (PIK3R3)ELISA Kit is to assay PIK3R3 levels in Mouse serum, plasma, culture media or any biological fluid.

 

Principle

 

This ELISA kit uses Sandwich-ELISA as the method. The Microelisa stripplate provided in this kit has been pre-coated with an antibody specific to PIK3R3. Standards or samples are added to the appropriate Microelisa stripplate wells and combined to the specific antibody. Then a Horseradish Peroxidase (HRP)- conjugated antibody specific for PIK3R3 is added to each Microelisa stripplate well and incubated. Free components are washed away. The TMB substrate solution is added to each well. Only those wells that contain PIK3R3 and HRP conjugated PIK3R3 antibody will appear blue in color and then turn yellow after the addition of the stop solution. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of PIK3R3. You can calculate the concentration of PIK3R3 in the samples by comparing the OD of the samples to the standard curve.

 

Typical Data

 

The standard curve of QS0896Mo is provided for demonstration only. A standard curve should be generated for each set of samples assayed.

 

 

 

 

Standard	Concentration	OD Value	Average OD Value
Blank Well	0ng/L	0.057	0.0550
		0.053
S1	240ng/L	1.968	1.9635
		1.959
S2	160ng/L	1.392	1.3385
		1.285
S3	80ng/L	0.710	0.7190
		0.728
S4	40ng/L	0.376	0.3855
		0.395
S5	20ng/L	0.205	0.1990
		0.193

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