Summary:

 

Purpose

 

Our Quick Step Bovine Glutathione Peroxidase GSH-PX ELISA Kit Enzyme activity is to assay GSH-PX levels in Bovine serum, plasma, culture media or any biological fluid.

 

Principle

 

This ELISA kit uses Sandwich-ELISA as the method. The Microelisa stripplate provided in this kit has been pre-coated with an antibody specific to GSH-PX. Standards or samples are added to the appropriate Microelisa stripplate wells and combined to the specific antibody. Then a Horseradish Peroxidase (HRP)- conjugated antibody specific for GSH-PX is added to each Microelisa stripplate well and incubated. Free components are washed away. The TMB substrate solution is added to each well. Only those wells that contain GSH-PX and HRP conjugated GSH-PX antibody will appear blue in color and then turn yellow after the addition of the stop solution. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of GSH-PX. You can calculate the concentration of GSH-PX in the samples by comparing the OD of the samples to the standard curve.

 

Typical Data

 

The standard curve of QS0024Bo is provided for demonstration only. A standard curve should be generated for each set of samples assayed.

 

 

 

 

Standard	Concentration	OD Value	Average OD Value
Blank Well	0IU/ml	0.057	0.0550
		0.053
S1	240IU/ml	1.968	1.9635
		1.959
S2	160IU/ml	1.392	1.3385
		1.285
S3	80IU/ml	0.710	0.7190
		0.728
S4	40IU/ml	0.376	0.3855
		0.395
S5	20IU/ml	0.205	0.1990
		0.193

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