Summary:

 

Purpose

 

Our Quick Step Human serine/threonine protein kinase,STK ELISA Kit is to assay STK levels in Human serum, plasma, culture media or any biological fluid.

 

Principle

 

This ELISA kit uses Sandwich-ELISA as the method. The Microelisa stripplate provided in this kit has been pre-coated with an antibody specific to STK. Standards or samples are added to the appropriate Microelisa stripplate wells and combined to the specific antibody. Then a Horseradish Peroxidase (HRP)- conjugated antibody specific for STK is added to each Microelisa stripplate well and incubated. Free components are washed away. The TMB substrate solution is added to each well. Only those wells that contain STK and HRP conjugated STK antibody will appear blue in color and then turn yellow after the addition of the stop solution. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of STK. You can calculate the concentration of STK in the samples by comparing the OD of the samples to the standard curve.

 

Typical Data

 

The standard curve of QS1569Hu is provided for demonstration only. A standard curve should be generated for each set of samples assayed.

 

 

 

 

Standard	Concentration	OD Value	Average OD Value
Blank Well	0ng/L	0.048	0.0500
		0.052
S1	300ng/L	1.939	1.9455
		1.952
S2	200ng/L	1.372	1.3865
		1.401
S3	100ng/L	0.722	0.7200
		0.718
S4	50ng/L	0.411	0.4035
		0.396
S5	25ng/L	0.201	0.2065
		0.212

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