Summary:

 

Purpose

 

Our Quick Step Human Cytokeratin 18-M65,CK 18-M65ELISA kit is to assay CK 18-M65 levels in Human serum, plasma, culture media or any biological fluid.

 

Principle

 

This ELISA kit uses Sandwich-ELISA as the method. The Microelisa stripplate provided in this kit has been pre-coated with an antibody specific to CK 18-M65. Standards or samples are added to the appropriate Microelisa stripplate wells and combined to the specific antibody. Then a Horseradish Peroxidase (HRP)- conjugated antibody specific for CK 18-M65 is added to each Microelisa stripplate well and incubated. Free components are washed away. The TMB substrate solution is added to each well. Only those wells that contain CK 18-M65 and HRP conjugated CK 18-M65 antibody will appear blue in color and then turn yellow after the addition of the stop solution. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of CK 18-M65. You can calculate the concentration of CK 18-M65 in the samples by comparing the OD of the samples to the standard curve.

 

Typical Data

 

The standard curve of QS0585Hu is provided for demonstration only. A standard curve should be generated for each set of samples assayed.

 

 

 

 

Standard	Concentration	OD Value	Average OD Value
Blank Well	0pg/m	0.048	0.0500
		0.052
S1	300pg/m	1.939	1.9455
		1.952
S2	200pg/m	1.372	1.3865
		1.401
S3	100pg/m	0.722	0.7200
		0.718
S4	50pg/m	0.411	0.4035
		0.396
S5	25pg/m	0.201	0.2065
		0.212

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