Summary:

 

Purpose

 

Our Quick Step Human tartrate-resistant acid phosphatase 5b,TRACP-5b ELISA Kit is to assay TRACP-5b levels in Human serum, plasma, culture media or any biological fluid.

 

Principle

 

This ELISA kit uses Sandwich-ELISA as the method. The Microelisa stripplate provided in this kit has been pre-coated with an 抗体QS1665Hu specific to TRACP-5b. Standards or samples are added to the appropriate Microelisa stripplate wells and combined to the specific 抗体QS1665Hu. Then a Horseradish Peroxidase (HRP)- conjugated 抗体QS1665Hu specific for TRACP-5b is added to each Microelisa stripplate well and incubated. Free components are washed away. The TMB substrate solution is added to each well. Only those wells that contain TRACP-5b and HRP conjugated TRACP-5b 抗体QS1665Hu will appear blue in color and then turn yellow after the addition of the stop solution. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of TRACP-5b. You can calculate the concentration of TRACP-5b in the samples by comparing the OD of the samples to the standard curve.

 

Typical Data

 

The standard curve of QS1665Hu is provided for demonstration only. A standard curve should be generated for each set of samples assayed.

 

 

 

 

Standard	Concentration	OD Value	Average OD Value
Blank Well	0ng/ml	0.040	0.0450
		0.050
S1	4.8ng/ml	1.958	1.9555
		1.953
S2	3.2ng/ml	1.308	1.3090
		1.310
S3	1.6ng/ml	0.698	0.7045
		0.711
S4	0.8ng/ml	0.397	0.3935
		0.390
S5	0.4ng/ml	0.209	0.2075
		0.206

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