Summary:

 

Purpose

 

Our Quick Step Human epididymal protein 4 (HE4)elisa Kit is to assay HE4 levels in Human serum, plasma, culture media or any biological fluid.

 

Principle

 

This ELISA kit uses Sandwich-ELISA as the method. The Microelisa stripplate provided in this kit has been pre-coated with an 抗体QS3431Hu specific to HE4. Standards or samples are added to the appropriate Microelisa stripplate wells and combined to the specific 抗体QS3431Hu. Then a Horseradish Peroxidase (HRP)- conjugated 抗体QS3431Hu specific for HE4 is added to each Microelisa stripplate well and incubated. Free components are washed away. The TMB substrate solution is added to each well. Only those wells that contain HE4 and HRP conjugated HE4 抗体QS3431Hu will appear blue in color and then turn yellow after the addition of the stop solution. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of HE4. You can calculate the concentration of HE4 in the samples by comparing the OD of the samples to the standard curve.

 

Typical Data

 

The standard curve of QS3431Hu is provided for demonstration only. A standard curve should be generated for each set of samples assayed.

 

 

 

 

Standard	Concentration	OD Value	Average OD Value
Blank Well	0ng/ml	0.040	0.0450
		0.050
S1	4.8ng/ml	1.958	1.9555
		1.953
S2	3.2ng/ml	1.308	1.3090
		1.310
S3	1.6ng/ml	0.698	0.7045
		0.711
S4	0.8ng/ml	0.397	0.3935
		0.390
S5	0.4ng/ml	0.209	0.2075
		0.206

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