Summary:

 

Purpose

 

Our Quick Step Mouse Glucose 6 Phosphate Dehydrogenase ( G6PD)ELISA Kit is to assay G6PD levels in Mouse serum, plasma, culture media or any biological fluid.

 

Principle

 

This ELISA kit uses Sandwich-ELISA as the method. The Microelisa stripplate provided in this kit has been pre-coated with an 抗体QS0684Mo specific to G6PD. Standards or samples are added to the appropriate Microelisa stripplate wells and combined to the specific 抗体QS0684Mo. Then a Horseradish Peroxidase (HRP)- conjugated 抗体QS0684Mo specific for G6PD is added to each Microelisa stripplate well and incubated. Free components are washed away. The TMB substrate solution is added to each well. Only those wells that contain G6PD and HRP conjugated G6PD 抗体QS0684Mo will appear blue in color and then turn yellow after the addition of the stop solution. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of G6PD. You can calculate the concentration of G6PD in the samples by comparing the OD of the samples to the standard curve.

 

Typical Data

 

The standard curve of QS0684Mo is provided for demonstration only. A standard curve should be generated for each set of samples assayed.

 

 

 

 

Standard	Concentration	OD Value	Average OD Value
Blank Well	0ng/ml	0.040	0.0450
		0.050
S1	4.8ng/ml	1.958	1.9555
		1.953
S2	3.2ng/ml	1.308	1.3090
		1.310
S3	1.6ng/ml	0.698	0.7045
		0.711
S4	0.8ng/ml	0.397	0.3935
		0.390
S5	0.4ng/ml	0.209	0.2075
		0.206

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