Summary:

 

Purpose

 

Our Quick Step Human Matrix metalloproteinase 2/Gelatinase A,MMP-2 ELISA Kit (Enzyme Active) is to assay MMP-2 levels in Human serum, plasma, culture media or any biological fluid.

 

Principle

 

This ELISA kit uses Sandwich-ELISA as the method. The Microelisa stripplate provided in this kit has been pre-coated with an antibody specific to MMP-2. Standards or samples are added to the appropriate Microelisa stripplate wells and combined to the specific antibody. Then a Horseradish Peroxidase (HRP)- conjugated antibody specific for MMP-2 is added to each Microelisa stripplate well and incubated. Free components are washed away. The TMB substrate solution is added to each well. Only those wells that contain MMP-2 and HRP conjugated MMP-2 antibody will appear blue in color and then turn yellow after the addition of the stop solution. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of MMP-2. You can calculate the concentration of MMP-2 in the samples by comparing the OD of the samples to the standard curve.

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