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Quick Step Human Hyaluronidase1 (HAase-1)elisa Kit
Quick Step Human Hyaluronidase1 (HAase-1)elisa Kit
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Regular members: $374.4
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  • NO.:QS2147Hu
    Sensitivity:0.01 ng/ml
    Assay range:0.1 ng/ml -15 ng/ml
    Standard:18ng/ml
    Sample Volume:10ul
    Wavelength:450nm
    Assay Time:60 minutes
  • Goods click count:116
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Details

 

Summary:

 

Purpose

 

Our Quick Step Human Hyaluronidase1 (HAase-1)elisa Kit is to assay HAase-1 levels in Human serum, plasma, culture media or any biological fluid.

 

Principle

 

This ELISA kit uses Sandwich-ELISA as the method. The Microelisa stripplate provided in this kit has been pre-coated with an antibody specific to HAase-1. Standards or samples are added to the appropriate Microelisa stripplate wells and combined to the specific antibody. Then a Horseradish Peroxidase (HRP)- conjugated antibody specific for HAase-1 is added to each Microelisa stripplate well and incubated. Free components are washed away. The TMB substrate solution is added to each well. Only those wells that contain HAase-1 and HRP conjugated HAase-1 antibody will appear blue in color and then turn yellow after the addition of the stop solution. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of HAase-1. You can calculate the concentration of HAase-1 in the samples by comparing the OD of the samples to the standard curve.

 

Typical Data

 

The standard curve of QS2147Hu is provided for demonstration only. A standard curve should be generated for each set of samples assayed.

 

 

 

 

Standard

Concentration

OD Value

Average OD Value

Blank Well

0ng/ml

0.063

0.0580

0.053

S1

12ng/ml

1.955

1.9585

1.962

S2

8ng/ml

1.288

1.2920

1.296

S3

4ng/ml

0.704

0.7080

0.712

S4

2ng/ml

0.387

0.3965

0.406

S5

1ng/ml

0.196

0.2000

0.204

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Wi***22024-09-06
Za***12024-09-03
Ya***22024-09-01
Et***22024-07-27
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