Summary:

 

Purpose

 

Our Quick Step Human poliovirus antibody Iga (PV-IgA)elisa Kit (Enzyme activity) is to assay PV-IgA levels in Human serum, plasma, culture media or any biological fluid.

 

Principle

 

This ELISA kit uses Sandwich-ELISA as the method. The Microelisa stripplate provided in this kit has been pre-coated with an antigen specific to PV-IgA. Standards or samples are added to the appropriate Microelisa stripplate wells and combined to the specific antigen. Then a Horseradish Peroxidase (HRP)- conjugated antigen specific for PV-IgA is added to each Microelisa stripplate well and incubated. Free components are washed away. The TMB substrate solution is added to each well. Only those wells that contain PV-IgA and HRP conjugated PV-IgA antigen will appear blue in color and then turn yellow after the addition of the stop solution. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of PV-IgA. You can calculate the concentration of PV-IgA in the samples by comparing the OD of the samples to the standard curve.

 

Typical Data

 

The standard curve of QS2736Hu_1 is provided for demonstration only. A standard curve should be generated for each set of samples assayed.

 

 

 

 

Standard	Concentration	OD Value	Average OD Value
Blank Well	0U/L	0.063	0.0580
		0.053
S1	12U/L	1.955	1.9585
		1.962
S2	8U/L	1.288	1.2920
		1.296
S3	4U/L	0.704	0.7080
		0.712
S4	2U/L	0.387	0.3965
		0.406
S5	1U/L	0.196	0.2000
		0.204

Cartpieces

• 
• 1

  1Delete

• 
• 8

  8Delete

• 
• Q

  QDelete

• 
• Q

  QDelete

• 
• +

  +Delete

• 
• 1

  1Delete

• 
• 2

  2Delete

• Delete

• 
• 1

  1Delete

• 
• 1

  1Delete

• 
• $

  $Delete

• 
• 0

  0Delete

• 
• 4

  4Delete

• 
• $

  $Delete

• 
• 0

  0Delete

• 
• 0

  0Delete

• 
• 1

  1Delete

• 
• Q

  QDelete

• Delete

• 
• <

  <Delete

• 
• i

  iDelete

• 
• i

  iDelete

• Delete

• 
• 1

  1Delete

• Delete

• 
• 1

  1Delete

• 
• 1

  1Delete

• 
• 0

  0Delete

• Delete

• 
• 2

  2Delete

• 
• 1

  1Delete

• 
• 0

  0Delete

• 
• 0

  0Delete

• 
• 0

  0Delete

• 
• 0

  0Delete

• 
• 0

  0Delete

• 
• 0

  0Delete

• 
• 1

  1Delete

• 
• 1

  1Delete

• Delete

• 
• -

  -Delete

• 
• -

  -Delete

• 
• 0

  0Delete

• Delete

• 
• t

  tDelete

• 
• 0

  0Delete

• 
• 0

  0Delete

• 
• 0

  0Delete

• 
• f

  fDelete

• Delete

• Delete

• Delete

• Delete

• Delete

• Delete

• Delete

• Delete

• Delete

• Delete

• Delete

• 
• S

  SDelete

• Delete

• Delete

• 
• 4

  4Delete

• 
• $

  $Delete

• Delete

• 
• 0

  0Delete

• Delete

• Delete

• Delete

• 
• b

  bDelete

• 
• Q

  QDelete

• 
• 8

  8Delete

• 
• Q

  QDelete

Totalgoods,subtotals:￥Checkout