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Quick Step Rabbit Insulin ,INS ELISA Kit
Quick Step Rabbit Insulin ,INS ELISA Kit
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Regular members: $398.4
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Details

 

Summary:

 

Purpose

 

Our Quick Step Rabbit Insulin,INS ELISA Kit is to assay INS levels in Rabbit serum, plasma, culture media or any biological fluid.

 

Principle

 

This ELISA kit uses Sandwich-ELISA as the method. The Microelisa stripplate provided in this kit has been pre-coated with an antibody specific to INS. Standards or samples are added to the appropriate Microelisa stripplate wells and combined to the specific antibody. Then a Horseradish Peroxidase (HRP)- conjugated antibody specific for INS is added to each Microelisa stripplate well and incubated. Free components are washed away. The TMB substrate solution is added to each well. Only those wells that contain INS and HRP conjugated INS antibody will appear blue in color and then turn yellow after the addition of the stop solution. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of INS. You can calculate the concentration of INS in the samples by comparing the OD of the samples to the standard curve.

 

Typical Data

 

The standard curve of QS0100Rb is provided for demonstration only. A standard curve should be generated for each set of samples assayed.

 

 

 

 

Standard

Concentration

OD Value

Average OD Value

Blank Well

0mU/L

0.063

0.0580

0.053

S1

12mU/L

1.955

1.9585

1.962

S2

8mU/L

1.288

1.2920

1.296

S3

4mU/L

0.704

0.7080

0.712

S4

2mU/L

0.387

0.3965

0.406

S5

1mU/L

0.196

0.2000

0.204

Partial purchase records(bought amounts latest6)

UsernameQuantitybought time
Ta***22024-08-27
Xa***32024-08-04
Ka***22024-06-15
Na***32024-05-06
Ha***22024-04-14
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