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Quick Step Mouse acetylcholine receptor antibody ,AChRab ELISA Kit
Quick Step Mouse acetylcholine receptor antibody ,AChRab ELISA Kit
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Details

 

Summary:

 

Purpose

 

Our Quick Step Mouse acetylcholine receptor antibody,AChRab ELISA Kit is to assay AChRab levels in Mouse serum, plasma, culture media or any biological fluid.

 

Principle

 

This ELISA kit uses Sandwich-ELISA as the method. The Microelisa stripplate provided in this kit has been pre-coated with an antigen specific to AChRab. Standards or samples are added to the appropriate Microelisa stripplate wells and combined to the specific antigen. Then a Horseradish Peroxidase (HRP)- conjugated antigen specific for AChRab is added to each Microelisa stripplate well and incubated. Free components are washed away. The TMB substrate solution is added to each well. Only those wells that contain AChRab and HRP conjugated AChRab antigen will appear blue in color and then turn yellow after the addition of the stop solution. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of AChRab. You can calculate the concentration of AChRab in the samples by comparing the OD of the samples to the standard curve.

 

Typical Data

 

The standard curve of QS0018Mo is provided for demonstration only. A standard curve should be generated for each set of samples assayed.

 

 

 

 

Standard

Concentration

OD Value

Average OD Value

Blank Well

0ng/ml

0.043

0.0460

0.049

S1

9ng/ml

1.933

1.9385

1.944

S2

6ng/ml

1.394

1.4015

1.409

S3

3ng/ml

0.713

0.7095

0.706

S4

1.5ng/ml

0.400

0.3970

0.394

S5

0.75ng/ml

0.206

0.2125

0.219

Partial purchase records(bought amounts latest17)

UsernameQuantitybought time
Ol***22024-09-03
Ch***12024-07-22
Um***32024-07-22
Ab***12024-06-24
Fi***32024-06-23
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