Summary:

 

Purpose

 

Our Quick Step mouse follicle-stimulating hormone,FSH ELISA Kit is to assay FSH levels in Mouse serum, plasma, culture media or any biological fluid.

 

Principle

 

This ELISA kit uses Sandwich-ELISA as the method. The Microelisa stripplate provided in this kit has been pre-coated with an antibody specific to FSH. Standards or samples are added to the appropriate Microelisa stripplate wells and combined to the specific antibody. Then a Horseradish Peroxidase (HRP)- conjugated antibody specific for FSH is added to each Microelisa stripplate well and incubated. Free components are washed away. The TMB substrate solution is added to each well. Only those wells that contain FSH and HRP conjugated FSH antibody will appear blue in color and then turn yellow after the addition of the stop solution. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of FSH. You can calculate the concentration of FSH in the samples by comparing the OD of the samples to the standard curve.

 

Typical Data

 

The standard curve of QS0223Mo is provided for demonstration only. A standard curve should be generated for each set of samples assayed.

 

 

 

 

Standard	Concentration	OD Value	Average OD Value
Blank Well	0IU/L	0.043	0.0460
		0.049
S1	9IU/L	1.933	1.9385
		1.944
S2	6IU/L	1.394	1.4015
		1.409
S3	3IU/L	0.713	0.7095
		0.706
S4	1.5IU/L	0.400	0.3970
		0.394
S5	0.75IU/L	0.206	0.2125
		0.219

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