Summary:

 

Purpose

 

Our Quick Step Human Free Tri-iodothyronine Indes,Free-T3 ELISA Kit is to assay Free-T3 levels in Human serum, plasma, culture media or any biological fluid.

 

Principle

 

This ELISA kit uses Sandwich-ELISA as the method. The Microelisa stripplate provided in this kit has been pre-coated with an antibody specific to Free-T3. Standards or samples are added to the appropriate Microelisa stripplate wells and combined to the specific antibody. Then a Horseradish Peroxidase (HRP)- conjugated antibody specific for Free-T3 is added to each Microelisa stripplate well and incubated. Free components are washed away. The TMB substrate solution is added to each well. Only those wells that contain Free-T3 and HRP conjugated Free-T3 antibody will appear blue in color and then turn yellow after the addition of the stop solution. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of Free-T3. You can calculate the concentration of Free-T3 in the samples by comparing the OD of the samples to the standard curve.

 

Typical Data

 

The standard curve of QS0736Hu is provided for demonstration only. A standard curve should be generated for each set of samples assayed.

 

 

 

 

Standard	Concentration	OD Value	Average OD Value
Blank Well	0pmol/L	0.043	0.0460
		0.049
S1	9pmol/L	1.933	1.9385
		1.944
S2	6pmol/L	1.394	1.4015
		1.409
S3	3pmol/L	0.713	0.7095
		0.706
S4	1.5pmol/L	0.400	0.3970
		0.394
S5	0.75pmol/L	0.206	0.2125
		0.219

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