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Quick Step Human heat shock protein 27 ,HSP-27 ELISA Kit
Quick Step Human heat shock protein 27 ,HSP-27 ELISA Kit
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Regular members: $374.4
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  • NO.:QS0827Hu
    Sensitivity:0.01 ng/ml
    Assay range:0.1 ng/ml -10 ng/ml
    Standard:13.5 ng/ml
    Sample Volume:10ul
    Wavelength:450nm
    Assay Time:60 minutes
  • Goods click count:188
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Details

 

Summary:

 

Purpose

 

Our Quick Step Human heat shock protein 27,HSP-27 ELISA Kit is to assay HSP-27 levels in Human serum, plasma, culture media or any biological fluid.

 

Principle

 

This ELISA kit uses Sandwich-ELISA as the method. The Microelisa stripplate provided in this kit has been pre-coated with an antibody specific to HSP-27. Standards or samples are added to the appropriate Microelisa stripplate wells and combined to the specific antibody. Then a Horseradish Peroxidase (HRP)- conjugated antibody specific for HSP-27 is added to each Microelisa stripplate well and incubated. Free components are washed away. The TMB substrate solution is added to each well. Only those wells that contain HSP-27 and HRP conjugated HSP-27 antibody will appear blue in color and then turn yellow after the addition of the stop solution. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of HSP-27. You can calculate the concentration of HSP-27 in the samples by comparing the OD of the samples to the standard curve.

 

Typical Data

 

The standard curve of QS0827Hu is provided for demonstration only. A standard curve should be generated for each set of samples assayed.

 

 

 

 

Standard

Concentration

OD Value

Average OD Value

Blank Well

0ng/ml

0.043

0.0460

0.049

S1

9ng/ml

1.933

1.9385

1.944

S2

6ng/ml

1.394

1.4015

1.409

S3

3ng/ml

0.713

0.7095

0.706

S4

1.5ng/ml

0.400

0.3970

0.394

S5

0.75ng/ml

0.206

0.2125

0.219

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