Summary:

 

Purpose

Our Quick Step Rat Brucella IgG ELISA Kit is to for the qualitative determination of Brucella IgG in Rat serum, plasma, culture media or any biological fluid.

 

Principle

This ELISA kit uses Sandwich-ELISA as the method. The Microelisa stripplate provided in this kit has been pre-coated with an antigen specific toBrucella-IgG. Standards or samples are added to the appropriate Microelisa stripplate wells and combined to the specific antigen. Then a Horseradish Peroxidase (HRP)-conjugated antigen specific for Brucella-IgG is added to each Microelisa stripplate well and incubated. Free components are washed away. The TMB substrate solution is added to each well. Only those wells that contain Brucella-IgG and HRP conjugated Brucella  antigen will appear blue in color and then turn yellow after the addition of the stop solution. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The presence of Brucella-IgG is determined by comparing with the CUT OFF value.

Determine the result
Test effectiveness: the average value of positive control ≥1.00; The average value of negative control ≤0.10. 
The critical value (CUT OFF) calculation: critical value = the average value of   negative control + 0.15
Negative judgement:if the OD value<CUT OFF, the sample is Rat Brucella IgG negative.
Positive judgement: if the OD value ≥CUT OFF,the sample is Rat Brucella IgG positive.

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