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Quick Step Human Butyric Acid(BA) ELISA kit
Quick Step Human Butyric Acid(BA) ELISA kit
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  • NO.:QS4289Hu
    Sensitivity:0.6 pg/ml
    Assay range:3 pg/ml -200 pg/ml
    Standard:270 pg/ml
    Sample Volume:10ul
    Wavelength:450nm
    Assay Time:60 minutes
  • Goods click count:57
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Details

 

Summary:

 

 

Purpose

 

Our Quick Step Human Butyric Acid(BA) ELISA kit is to assay BA levels in Human serum, plasma, culture media or any biological fluid.

 

Principle

 

This ELISA kit uses Sandwich-ELISA as the method. The Microelisa stripplate provided in this kit has been pre-coated with an antibody specific to BA. Standards or samples are added to the appropriate Microelisa stripplate wells and combined to the specific antibody. Then a Horseradish Peroxidase (HRP)- conjugated antibody specific for BA is added to each Microelisa stripplate well and incubated. Free components are washed away. The TMB substrate solution is added to each well. Only those wells that contain BA and HRP conjugated BA antibody will appear blue in color and then turn yellow after the addition of the stop solution. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of BA. You can calculate the concentration of BA in the samples by comparing the OD of the samples to the standard curve.

 

Typical Data

 

The standard curve of QS4289Hu is provided for demonstration only. A standard curve should be generated for each set of samples assayed.

 

 

 

 

Standard

Concentration

OD Value

Average OD Value

Blank Well

0pg/ml

0.048

0.0505

0.053

S1

180pg/ml

1.941

1.9370

1.933

S2

120pg/ml

1.317

1.3255

1.334

S3

60pg/ml

0.709

0.7200

0.731

S4

30pg/ml

0.373

0.3645

0.356

S5

15pg/ml

0.187

0.1900

0.193

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