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Quick Step Human Peptidase D/Prolidase , PEPD ELISA Kit (Enzyme activity)
Quick Step Human Peptidase D/Prolidase , PEPD ELISA Kit (Enzyme activity)
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  • NO.:QS2456Hu_1
    Sensitivity:0.06 u/ml
    Assay range:0.3 u/ml-20 u/ml
    Standard:27.0 u/ml
    Sample Volume:10ul
    Wavelength:450nm
    Assay Time:60 minutes
  • Goods click count:56
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Details

 

Summary:

 

 

 

Purpose

Our Human Peptidase D/Prolidase (PEPD)ELISA Kit (Enzyme activity) is to assay PEPD levels in Human serum, plasma, culture media or any biological fluid.

Principle

This ELISA kit uses Sandwich-ELISA as the method. The Microelisa stripplate provided in this kit has been pre-coated with an antibody specific to PEPD. Standards or samples are added to the appropriate Microelisa stripplate wells and combined to the specific antibody. Then a Horseradish Peroxidase (HRP)- conjugated antibody specific for PEPD is added to each Microelisa stripplate well and incubated. Free components are washed away. The TMB substrate solution is added to each well. Only those wells that contain PEPD and HRP conjugated PEPD antibody will appear blue in color and then turn yellow after the addition of the stop solution. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of PEPD. You can calculate the concentration of PEPD in the samples by comparing the OD of the samples to the standard curve.

 

Typical Data

The standard curve of QS2456Hu_1 is provided for demonstration only. A standard curve should be generated for each set of samples assayed. 

 

 

 

 

Standard

Concentration

OD Value

Average OD Value

Blank Well

0u/ml

0.048

0.0465

0.045

S1

18u/ml

1.973

1.9760

1.979

S2

12u/ml

1.363

1.3675

1.372

S3

6u/ml

0.725

0.7220

0.719

S4

3u/ml

0.388

0.3860

0.384

S5

1.5u/ml

0.187

0.1895

0.192

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