Drug Names

Generic Name: Canine Cathepsin B (Cath-B)ELISA Kit

Purpose

Our Canine Cathepsin B (Cath-B)ELISA kit is to assay Cath-B levels in Canine serum, plasma, culture media or any biological fluid.

Principle

This ELISA kit uses Sandwich-ELISA as the method. The Microelisa stripplate provided in this kit has been pre-coated with an antibody specific to Cath-B, Standards or samples are added to the appropriate Microelisa stripplate wells and combined to the specific antibody. Then a Horseradish Peroxidase (HRP)-conjugated antibody specific for Cath-B is added to each Microelisa stripplate well and incubated. Free components are washed away. The TMB substrate solution is added to each well. Only those wells that contain Cath-B and HRP conjugated Cath-B antibody will appear blue in color and then turn yellow after the addition of the stop solution. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of Cath-B. You can calculate the concentration of Cath-B in the samples by comparing the OD of the samples to the standard curve.

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