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Quick Step Goat Interleukin 2 (IL-5)ELISA Kit
Quick Step Goat Interleukin 2 (IL-5)ELISA Kit
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Regular members: $422.4
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  • NO.:QS0112Gt
    Assay range:3pg/ml-200pg/ml
    Sensitivity:0.5pg/ml
    Standard:270.0pg/ml
    Sample Volume:10ul
    Wavelength:450nm
    Assay Time:60 minutes
  • Goods click count:49
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Details

Purpose

Our Quick Step Goat Interleukin 2 (IL-5)ELISA Kit is to assay IL-5 levels in Goat serum, plasma, culture media or any biological fluid.

Principle

This ELISA kit uses Sandwich-ELISA as the method. The Microelisa stripplate provided in this kit has been pre-coated with an antibody specific to IL-5. Standards or samples are added to the appropriate Microelisa stripplate wells and combined to the specific antibody. Then a Horseradish Peroxidase (HRP)- conjugated antibody specific for IL-5 is added to each Microelisa stripplate well and incubated. Free components are washed away. The TMB substrate solution is added to each well. Only those wells that contain IL-5 and HRP conjugated IL-5 antibody will appear blue in color and then turn yellow after the addition of the stop solution. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of IL-5. You can calculate the concentration of IL-5 in the samples by comparing the OD of the samples to the standard curve.

Typical Data

 

The standard curve of QS0112Gt is provided for demonstration only. A standard curve should be generated for each set of samples assayed.

 

 

Standard

Concentration

OD Value

Average OD Value

Blank Well

0

0.048

0.0505

0.053

S1

180

1.941

1.9370

1.933

S2

120

1.317

1.3255

1.334

S3

60

0.709

0.7200

0.731

S4

30

0.373

0.3645

0.356

S5

15

0.187

0.1900

0.193

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