Drug Names

Generic Name: Mouse Nicotinamide Adenine Dinucleotide Phosphate (NADPH)ELISA Kit

Purpose

Our Mouse Nicotinamide Adenine Dinucleotide Phosphate (NADPH)ELISA kit is to assay NADPH levels in Mouse serum, plasma, culture media or any biological fluid.

Principle

This ELISA kit uses Sandwich-ELISA as the method. The Microelisa stripplate provided in this kit has been pre-coated with an antibody specific to NADPH. Standards or samples are added to the appropriate Microelisa stripplate wells and combined to the specific antibody. Then a Horseradish Peroxidase (HRP)-conjugated antibody specific for NADPH is added to each Microelisa stripplate well and incubated. Free components are washed away. The TMB substrate solution is added to each well. Only those wells that contain NADPH and HRP conjugated NADPH antibody will appear blue in color and then turn yellow after the addition of the stop solution. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of NADPH. You can calculate the concentration of NADPH in the samples by comparing the OD of the samples to the standard curve.

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