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Summary:
Purpose
Our Quick Step Human Granzyme (GZM)ELISA Kit is to assay GZM levels in Human serum, plasma, culture media or any biological fluid.
Principle
This ELISA kit uses Sandwich-ELISA as the method. The Microelisa stripplate provided in this kit has been pre-coated with an antibody specific to GZM. Standards or samples are added to the appropriate Microelisa stripplate wells and combined to the specific antibody. Then a Horseradish Peroxidase (HRP)-conjugated antibody specific for GZM is added to each Microelisa stripplate well and incubated. Free components are washed away. The TMB substrate solution is added to each well. Only those wells that contain GZM and HRP conjugated GZM antibody will appear blue in color and then turn yellow after the addition of the stop solution. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of GZM. You can calculate the concentration of GZM in the samples by comparing the OD of the samples to the standard curve.
Typical Data
The standard curve of QS4318Hu is provided for demonstration only. A standard curve should be generated for each set of samples assayed.
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Standard
Concentration
OD Value
Average OD Value
Blank Well
0
0.042
0.0485
0.055
S1
120
1.998
1.9855
1.973
S2
80
1.318
1.3240
1.330
S3
40
0.688
0.6915
0.695
S4
20
0.393
0.3875
0.382
S5
10
0.201
0.1950
0.189
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