Summary:

 

Purpose

Our Quick Step Human Anapastic Lymphoma Kinase (ALK)ELISA Kit is to assay ALK levels in Human serum, plasma, culture media or any biological fluid.

Principle

This ELISA kit uses Sandwich-ELISA as the method. The Microelisa stripplate provided in this kit has been pre-coated with an antibody specific to ALK. Standards or samples are added to the appropriate Microelisa stripplate wells and combined to the specific antibody. Then a Horseradish Peroxidase (HRP)-conjugated antibody specific for ALK is added to each Microelisa stripplate well and incubated. Free components are washed away. The TMB substrate solution is added to each well. Only those wells that contain ALK and HRP conjugated ALK antibody will appear blue in color and then turn yellow after the addition of the stop solution. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of ALK. You can calculate the concentration of ALK in the samples by comparing the OD of the samples to the standard curve.

Typical Data

 

The standard curve of QS0105Hu is provided for demonstration only. A standard curve should be generated for each set of samples assayed.

 

Standard	Concentration	OD Value	Average OD Value
Blank Well	0pg/ml	0.033	0.0360
		0.039
S1	900pg/ml	1.985	1.9810
		1.977
S2	600pg/ml	1.363	1.3695
		1.376
S3	300pg/ml	0.752	0.7480
		0.744
S4	150pg/ml	0.384	0.3805
		0.377
S5	75pg/ml	0.169	0.1780
		0.187

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