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Drug Names
Generic Name: Avian Influenza Viruses A (FLU A)ELISA Kit
Purpose
Our Avian Influenza Viruses A (FLU A)ELISA kit is for the qualitative determination of FLU A in Avian serum, plasma, culture media or any biological fluid.
Principle
The ELISA is based on the the qualitative enzyme immunoassay technique.The Microplate provided in this kit has been pre-coated with an Antibody specific to FLU A, make it to solid-phase antibody.Samples are added to the Microplate wells and combined to the specific antibody. Then a Horseradish Peroxidase (HRP)-conjugated antibody specific for FLU A is added to each Microplate well and incubated,so the antibody-antigen-Enzyme labeled antibody complex is formed.Following a wash to remove any unbound reagent,then the TMB substrate solution is added to each well. Only those wells that contain FLU A and HRP conjugated FLU A antibody will appear blue in color and then turn yellow after the addition of the stop solution. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The qualitative determination of FLU A is determined by comparing with the CUTOFF value.
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