Summary:

Purpose

Our Quick Step Human 4-Hydroxynonenal (4-HNE)ELISA Kit is to assay 4-HNE levels in Human serum, plasma, culture media or any biological fluid.

 

Principle

This ELISA kit uses Sandwich-ELISA as the method. The Microelisa stripplate provided in this kit has been pre-coated with an antibody specific to 4-HNE. Standards or samples are added to the appropriate Microelisa stripplate wells and combined to the specific antibody. Then a Horseradish Peroxidase (HRP)-conjugated antibody specific for 4-HNE is added to each Microelisa stripplate well and incubated. Free components are washed away. The TMB substrate solution is added to each well. Only those wells that contain 4-HNE and HRP conjugated 4-HNE antibody will appear blue in color and then turn yellow after the addition of the stop solution. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of 4-HNE. You can calculate the concentration of 4-HNE in the samples by comparing the OD of the samples to the standard curve.

Typical Data

 The standard curve of QS4414Hu is provided for demonstration only. A standard curve should be generated for each set of samples assayed.

 

Standard	Concentration	OD Value	Average OD Value
Blank Well	0ng/ml	0.060	0.0560
		0.052
S1	7.2ng/ml	1.960	1.9575
		1.955
S2	4.8ng/ml	1.292	1.2875
		1.283
S3	2.4ng/ml	0.701	0.7045
		0.708
S4	1.2ng/ml	0.391	0.3920
		0.393
S5	0.6ng/ml	0.212	0.2045
		0.197

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