Summary:

Purpose

Our Quick Step Human Irisin ELISA Kit is to assay Irisin levels in Human serum, plasma, culture media or any biological fluid.

 

Principle

This ELISA kit uses Sandwich-ELISA as the method. The Microelisa stripplate provided in this kit has been pre-coated with an antibody specific to Irisin. Standards or samples are added to the appropriate Microelisa stripplate wells and combined to the specific antibody. Then a Horseradish Peroxidase (HRP)-conjugated antibody specific for Irisin is added to each Microelisa stripplate well and incubated. Free components are washed away. The TMB substrate solution is added to each well. Only those wells that contain Irisin and HRP conjugated Irisin antibody will appear blue in color and then turn yellow after the addition of the stop solution. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of Irisin. You can calculate the concentration of Irisin in the samples by comparing the OD of the samples to the standard curve.

Typical Data

 The standard curve of QS4422Hu is provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Standard	Concentration	OD Value	Average OD Value
Blank Well	0pg/ml	0.047	0.0400
		0.033
S1	720pg/ml	1.958	1.9635
		1.969
S2	480pg/ml	1.393	1.3900
		1.387
S3	240pg/ml	0.715	0.7225
		0.730
S4	120pg/ml	0.375	0.3815
		0.388
S5	60pg/ml	0.196	0.1985
		0.201

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