Summary:

Purpose

Our Quick Step Human Interleukin 13 (IL-13)ELISA Kit is to assay IL-13 levels in Human serum, plasma, culture media or any biological fluid.

 

Principle

This ELISA kit uses Sandwich-ELISA as the method. The Microelisa stripplate provided in this kit has been pre-coated with an antibody specific to IL-13. Standards or samples are added to the appropriate Microelisa stripplate wells and combined to the specific antibody. Then a Horseradish Peroxidase (HRP)-conjugated antibody specific for IL-13 is added to each Microelisa stripplate well and incubated. Free components are washed away. The TMB substrate solution is added to each well. Only those wells that contain IL-13 and HRP conjugated IL-13 antibody will appear blue in color and then turn yellow after the addition of the stop solution. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of IL-13. You can calculate the concentration of IL-13 in the samples by comparing the OD of the samples to the standard curve.

Typical Data

 The standard curve of CQS0974Hu is provided for demonstration only. A standard curve should be generated for each set of samples assayed. 

 

Standard	Concentration	OD Value	Average OD Value
Blank Well	0	0.057	0.0550
		0.053
S1	240	1.968	1.9635
		1.959
S2	160	1.392	1.3385
		1.285
S3	80	0.710	0.7190
		0.728
S4	40	0.376	0.3855
		0.395
S5	20	0.205	0.1990
		0.193

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