Quantitative analysis of DNA residue in recombinant protein expressed products, purified intermediate and finished products from host cell..

 

**1.Introduction**

 

This kit adopts Taqman probe fluorescence qPCR method. The kit has the advantages of high specificity and sensitivity by using specific primers & probes, LOQ can reach 30fg/μL level. This kit can be used in combination with our Magnetic Residual DNA Sample Preparation Kit (Cat#CG-HCD100T). The preparation process of DNA Control is completely consistent with that of other host cell DNA national standards, therefore it has high purity and no protein and ion interference to ensure the accuracy of the sample quantitative detection.

 

The kit provides DNA Dilution Buffer, which enables good replicate parallelism in a single experiment and good reproducibility between multiple experiments.

 

 **2. MATERIALS**

 

DNA Amplification
Components NO.	Components Name	Size
		50T	100T
B1	2X qPCR Mix(UDG plus)	0.76mL	1.52mL
B2	Primer&Probe Mix	100μL	200μL
B3	DNA Dilution Buffer	2×1.5mL	4×1.5mL
B4	DNA Control (30ng/μL)	25μL	50μL
B5	RNase-Free H2O	0.5mL	1mL
B6	50X ROX Reference Dye(Optional)	0.15ml	0.3ml

 **3.Shipping and Storage**

• All components are shipped on dry ice or ice bags.
• The kit should be stored at -20℃ and it is recommended to be used within one year. B2 should be stored protected from light.
• B2/B3/B4 can be stored at -20℃ for 2 years, while B1/B5/B6 can be stored at -20℃ for 1 year. B1/B5/B6 can also be purchased together as a separate set.

 

 

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