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Quantitative analysis of DNA residue in recombinant protein expressed products, purified intermediate and finished products from host cell..
**1.Introduction**
This kit adopts Taqman probe fluorescence qPCR method. The kit has the advantages of high specificity and sensitivity by using specific primers & probes, LOQ can reach 10fg/μL level. This kit can be used in combination with our Magnetic Residual DNA Sample Preparation Kit (Cat#CG-DP100). The preparation process of DNA Control is completely consistent with that of other host cell DNA national standards, therefore it has high purity and no protein and ion interference to ensure the accuracy of the sample quantitative detection.
The kit provides DNA Dilution Buffer, which enables good replicate parallelism in a single experiment and good reproducibility between multiple experiments.
**2. MATERIALS**
DNA Amplification
Components NO.
Components Name
Size
50T
100T
B1
2XqPCR Mix
0.625mL
1.25mL
B2
Primer&Probe Mix
100μL
200μL
B3
2×1.5mL
4×1.5mL
B4
DNA Control (10ng/μL)
25μL
50μL
B5
RNase-Free H2O
0.5mL
1mL
B6
50X ROX Reference Dye(Optional)
0.15ml
0.3ml
**3.Shipping and Storage**
- All components are shipped on dry ice or ice bags.
- The kit should be stored at -20℃ and it is recommended to be used within one year. B2 should be stored protected from light.
- B2/B3/B4 can be stored at -20℃ for 2 years, while B1/B5/B6 can be stored at -20℃ for 1 year. B1/B5/B6 can also be purchased together as a separate set.
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