Summary:

 Purpose

Our Quick Step Bovine heart Fatty Acid Binding Protein (h-FABP)ELISA Kit is to assay h-FABP levels in Bovine serum, plasma, culture media or any biological fluid.

 

Principle

This ELISA kit uses Sandwich-ELISA as the method. The Microelisa stripplate provided in this kit has been pre-coated with an antibody specific to h-FABP. Standards or samples are added to the appropriate Microelisa stripplate wells and combined to the specific antibody. Then a Horseradish Peroxidase (HRP)-conjugated antibody specific for h-FABP is added to each Microelisa stripplate well and incubated. Free components are washed away. The TMB substrate solution is added to each well. Only those wells that contain h-FABP and HRP conjugated h-FABP antibody will appear blue in color and then turn yellow after the addition of the stop solution. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of h-FABP. You can calculate the concentration of h-FABP in the samples by comparing the OD of the samples to the standard curve.

Typical Data

The standard curve of QS0333Bo is provided for demonstration only. A standard curve should be generated for each set of samples assayed. 

Standard	Concentration	OD Value	Average OD Value
Blank Well	0ng/ml	0.060	0.0560
		0.052
S1	7.2ng/ml	1.960	1.9575
		1.955
S2	4.8ng/ml	1.292	1.2875
		1.283
S3	2.4ng/ml	0.701	0.7045
		0.708
S4	1.2ng/ml	0.391	0.3920
		0.393
S5	0.6ng/ml	0.212	0.2045
		0.197

 

 

Cartpieces

• 
• 1

  1Delete

• 
• 8

  8Delete

• 
• Q

  QDelete

• 
• Q

  QDelete

• 
• +

  +Delete

• 
• 8

  8Delete

• 
• 2

  2Delete

• Delete

• 
• 2

  2Delete

• 
• 1

  1Delete

• 
• $

  $Delete

• 
• 0

  0Delete

• 
• 5

  5Delete

• 
• $

  $Delete

• 
• 0

  0Delete

• 
• 0

  0Delete

• 
• 1

  1Delete

• 
• Q

  QDelete

• Delete

• 
• <

  <Delete

• 
• i

  iDelete

• 
• i

  iDelete

• Delete

• 
• 1

  1Delete

• Delete

• 
• 1

  1Delete

• 
• 1

  1Delete

• 
• 0

  0Delete

• Delete

• 
• 2

  2Delete

• 
• 1

  1Delete

• 
• 0

  0Delete

• 
• 0

  0Delete

• 
• 0

  0Delete

• 
• 0

  0Delete

• 
• 0

  0Delete

• 
• 0

  0Delete

• 
• 1

  1Delete

• 
• 1

  1Delete

• Delete

• 
• -

  -Delete

• 
• -

  -Delete

• 
• 0

  0Delete

• Delete

• Delete

• 
• 0

  0Delete

• 
• 0

  0Delete

• 
• 0

  0Delete

• 
• f

  fDelete

• Delete

• Delete

• 
• 0

  0Delete

• 
• 0

  0Delete

• Delete

• Delete

• Delete

• Delete

• Delete

• Delete

• Delete

• 
• S

  SDelete

• Delete

• Delete

• 
• 5

  5Delete

• 
• $

  $Delete

• Delete

• 
• 0

  0Delete

• Delete

• Delete

• Delete

• 
• b

  bDelete

• 
• Q

  QDelete

• 
• 8

  8Delete

• 
• Q

  QDelete

Totalgoods,subtotals:￥Checkout