Summary:

 

Purpose

Our Quick Step Rat Vitamin C (VC)ELISA Kit is to assay VC levels in Rat serum, plasma, culture media or any biological fluid.

 

Principle

This ELISA kit uses Sandwich-ELISA as the method. The Microelisa stripplate provided in this kit has been pre-coated with an antibody specific to VC. Standards or samples are added to the appropriate Microelisa stripplate wells and combined to the specific antibody. Then a Horseradish Peroxidase (HRP)-conjugated antibody specific for VC is added to each Microelisa stripplate well and incubated. Free components are washed away. The TMB substrate solution is added to each well. Only those wells that contain VC and HRP conjugated VC antibody will appear blue in color and then turn yellow after the addition of the stop solution. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of VC. You can calculate the concentration of VC in the samples by comparing the OD of the samples to the standard curve.

Typical Data

The standard curve of QS0751Ra is provided for demonstration only. A standard curve should be generated for each set of samples assayed. 

 

Standard	Concentration	OD Value	Average OD Value
Blank Well	0ug/ml	0.043	0.0475
		0.052
S1	72ug/ml	1.988	1.9805
		1.973
S2	48ug/ml	1.302	1.3095
		1.317
S3	24ug/ml	0.692	0.7035
		0.715
S4	12ug/ml	0.377	0.3855
		0.394
S5	6ug/ml	0.202	0.1980
		0.194

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