Summary:

Purpose

Our Quick Step Human Claudin 25 (CLDN25)ELISA Kit is to assay CLDN25 levels in Human serum, plasma, culture media or any biological fluid.

 

Principle

This ELISA kit uses Sandwich-ELISA as the method. The Microelisa stripplate provided in this kit has been pre-coated with an antibody specific to CLDN25. Standards or samples are added to the appropriate Microelisa stripplate wells and combined to the specific antibody. Then a Horseradish Peroxidase (HRP)-conjugated antibody specific for CLDN25 is added to each Microelisa stripplate well and incubated. Free components are washed away. The TMB substrate solution is added to each well. Only those wells that contain CLDN25 and HRP conjugated CLDN25 antibody will appear blue in color and then turn yellow after the addition of the stop solution. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of CLDN25. You can calculate the concentration of CLDN25 in the samples by comparing the OD of the samples to the standard curve.

Typical Data

 The standard curve of QS4484Hu is provided for demonstration only. A standard curve should be generated for each set of samples assayed. 

Standard	Concentration	OD Value	Average OD Value
Blank Well	0pg/ml	0.049	0.0440
		0.039
S1	1800pg/ml	1.956	1.9530
		1.950
S2	1200pg/ml	1.389	1.3910
		1.393
S3	600pg/ml	0.709	0.7135
		0.718
S4	300pg/ml	0.390	0.3875
		0.385
S5	150pg/ml	0.195	0.2000
		0.205

 

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