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Quick Step Mouse Glutamate Cysteine Ligase, Catalytic (GCLC)ELISA Kit
Quick Step Mouse Glutamate Cysteine Ligase, Catalytic (GCLC)ELISA Kit
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  • NO.:QS1065Mo
    Wavelength:450nm
    Sample Volume:10ul
    Standard:5400pg/ml
    Assay range:44.8pg/ml-4000pg/ml
    Assay Time:60 minutes
    Sensitivity:8.9pg/ml
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Details

Summary:

 

Purpose

Our Quick Step Mouse Glutamate Cysteine Ligase, Catalytic (GCLC)ELISA Kit is to assay GCLC levels in Mouse serum, plasma, culture media or any biological fluid.

 

Principle

This ELISA kit uses Sandwich-ELISA as the method. The Microelisa stripplate provided in this kit has been pre-coated with an antibody specific to GCLC. Standards or samples are added to the appropriate Microelisa stripplate wells and combined to the specific antibody. Then a Horseradish Peroxidase (HRP)-conjugated antibody specific for GCLC is added to each Microelisa stripplate well and incubated. Free components are washed away. The TMB substrate solution is added to each well. Only those wells that contain GCLC and HRP conjugated GCLC antibody will appear blue in color and then turn yellow after the addition of the stop solution. The optical density (OD) is measured spectrophotomeGCLCally at a wavelength of 450 nm. The OD value is proportional to the concentration of GCLC. You can calculate the concentration of GCLC in the samples by comparing the OD of the samples to the standard curve.

Typical Data

 The standard curve of QS1065Mo is provided for demonstration only. A standard curve should be generated for each set of samples assayed. 

Standard

Concentration

OD Value

Average OD Value

Blank Well

0pg/ml

0.052

0.0460

0.040

S1

3600pg/ml

1.912

1.9075

1.903

S2

2400pg/ml

1.388

1.3840

1.380

S3

1200pg/ml

0.708

0.7100

0.712

S4

600pg/ml

0.403

0.4000

0.397

S5

300pg/ml

0.189

0.1960

0.203

 

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