Aliases:	ACSL4; Acyl-CoA Synthetase Long Chain Family Member 4; LACS4; ACS4; FACL4; Fatty-Acid-Coenzyme A Ligase, Long-Chain 4; Long-Chain Fatty-Acid-Coenzyme A Ligase 4; Long-Chain-Fatty-Acid--CoA Ligase 4; Long-Chain Acyl-CoA Synthetase 4; Lignoceroyl-CoA Synthase; Arachidonate--CoA Ligase; EC 6.2.1.3; MRX63; MRX68; Mental Retardation, X-Linked 63; Mental Retardation, X-Linked 68; Acyl-CoA Synthetase 4; EC 6.2.1.15; XLID63; LACS 4
Background:	UniProt Entry: O60488;NCBI Gene Entry: 2182
Application Information	Molecular Weight: Predicted, 79 kDa, observed, 73 kDa Clonality: Rabbit monoclonal antibody Clone ID: 23GB2960 Species Reactivity: Human, Mouse, Rat Applications Tested: Western Blotting (WB), Flow Cytometry (FCM), Immunocytochemistry (IC)
Immunogen	A synthesized peptide derived from ACSL4
Isotype	Rabbit IgG
Storage Buffer	Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage	Store at -20 °C for one year.
Recommended Dilutions	Western Blotting (WB): 1:1,000-1:5,000 Flow Cytometry (FCM): 1:2,000 Immunocytochemistry (IC): 1:1,000
Protocols	For general and specific antibody protocols please visit our website. Read all instructions before using this product.

Flow cytometric analysis of ACSL4 expression in HT-1080 cells using ACSL4 antibody 1:2,000. Green, isotype control; red, ACSL4.

Immunocytochemical staining of HT-1080 cells with ACSL4 antibody 1:1,000. Nuclei were stained blue with DAPI; ACSL4 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.

Western blotting analysis using anti-ACSL4 antibody 1:5,000 and HRP-conjugated goat anti-rabbit secondary antibody 1:20,000 respectively. ACSL4, acyl-CoA synthetase long chain family member 4.

Western blotting analysis using anti-ACSL4 antibody 1:5,000 and HRP-conjugated goat anti-rabbit secondary antibody 1:20,000 respectively.

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