Drug Names

Generic Name：Human Eukaryotic Translation Initiation Factor 4E (EIF4E)ELISA Kit

Purpose

Our Human Eukaryotic Translation Initiation Factor 4E (EIF4E)ELISA kit is to assay EIF4E levels in Human serum, plasma, culture media or any biological fluid.

Principle

This ELISA kit uses Sandwich-ELISA as the method. The Microelisa stripplate provided in this kit has been pre-coated with an antibody specific to EIF4E Standards or samples are added to the appropriate Microelisa stripplate wells and combined to the specific antibody. Then a Horseradish Peroxidase (HRP)-conjugated antibody specific for EIF4E is added to each Microelisa stripplate well and incubated. Free components are washed away. The TMB substrate solution is added to each well. Only those wells that contain EIF4E and HRP conjugated EIF4E antibody will appear blue in color and then turn yellow after the addition of the stop solution. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of EIF4E You can calculate the concentration of EIF4E in the samples by comparing the OD of the samples to the standard curve.

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