Drug Names

Generic Name: Human Chlamydia Trachomatis IgG (CT-IgM)ELISA Kit with Calibrator

Purpose

Our Human Chlamydia Trachomatis IgG (CT-IgM)ELISA Kit is to for the qualitative determination of CT-IgM  in Human serum, plasma, culture media or any biological fluid.

Principle

The ELISA is based on the the qualitative enzyme immunoassay technique. The Microplate provided in this kit has been pre-coated with an antigen specific to CT-IgM, make it to solid-phase antigen. Samples are added to the Microplate wells and combined to the specific antigen. Then a Horseradish Peroxidase (HRP)-conjugated antigen specific for CT is added to each Microplate well and incubated, so the antigen-antibody-Enzyme labeled antigen complex is formed. Following a wash to remove any unbound reagent, then the TMB substrate solution is added to each well. Only those wells that contain CT-IgM and HRP conjugated CT antigen will appear blue in color and then turn yellow after the addition of the stop solution. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The qualitative determination of CT-IgM is determined by comparing with the CUTOFF value.

Materials provided with the kit

 

	Materials provided with the kit	96 determinations	Storage
1	User manual	1	R.T.
2	Closure plate membrane	2	R.T.
3	Sealed bags	1	R.T.
4	Microplate	1	2-8℃
5	Calibrator	0.5ml×1 bottle	2-8℃
6	Negative control	0.5ml×1 bottle	2-8℃
7	Positive control	0.5ml×1 bottle	2-8℃
8	HRP-Conjugate reagent	6ml×1 bottle	2-8℃
9	Sample diluent	6ml×1 bottle	2-8℃
10	Chromogen Solution A	6ml×1 bottle	2-8℃
11	Chromogen Solution B	6ml×1 bottle	2-8℃
12	Stop Solution	6ml×1 bottle	2-8℃
13	wash solution	20ml (30X)×1bottle	2-8℃

 

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