Summary:

Purpose

Our Quick Step Human Ferredoxin 1 (FDX1)ELISA Kit is to assay FDX1 levels in Human serum, plasma, culture media or any biological fluid.

 

Principle

This ELISA kit uses Sandwich-ELISA as the method. The Microelisa stripplate provided in this kit has been pre-coated with an antibody specific to FDX1. Standards or samples are added to the appropriate Microelisa stripplate wells and combined to the specific antibody. Then a Horseradish Peroxidase (HRP)-conjugated antibody specific for FDX1 is added to each Microelisa stripplate well and incubated. Free components are washed away. The TMB substrate solution is added to each well. Only those wells that contain FDX1 and HRP conjugated FDX1 antibody will appear blue in color and then turn yellow after the addition of the stop solution. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of FDX1. You can calculate the concentration of FDX1 in the samples by comparing the OD of the samples to the standard curve.

Typical Data

 The standard curve of QS4561Hu is provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Standard	Concentration	OD Value	Average OD Value
Blank Well	0	0.044	0.0480
		0.052
S1	150	1.967	1.9630
		1.959
S2	100	1.328	1.3195
		1.311
S3	50	0.692	0.7030
		0.714
S4	25	0.392	0.3875
		0.383
S5	12.5	0.197	0.1900
		0.183

 

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