Drug Names

Generic Name: Human Purinergic Receptor P2X, Ligand Gated Ion Channel 7

(P2RX7)ELISA Kit

Purpose

Our Human Purinergic Receptor P2X, Ligand Gated Ion Channel 7 (P2RX7)ELISA kit is to assay P2RX7 levels in Human serum, plasma, culture media or any biological fluid.

Principle

This ELISA kit uses Sandwich-ELISA as the method. The Microelisa stripplate provided in this kit has been pre-coated with an antibody specific to P2RX7. Standards or samples are added to the appropriate Microelisa stripplate wells and combined to the specific antibody. Then a Horseradish Peroxidase (HRP)-conjugated antibody specific for P2RX7 is added to each Microelisa stripplate well and incubated. Free components are washed away. The TMB substrate solution is added to each well. Only those wells that contain P2RX7 and HRP conjugated P2RX7 antibody will appear blue in color and then turn yellow after the addition of the stop solution. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of P2RX7. You can calculate the concentration of P2RX7 in the samples by comparing the OD of the samples to the standard curve.

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