TEL: +86 571 56623320 EMAIL: [email protected]
1. Purpose
This kit is Fumonisin B1(FB1)Quantitative detection of residues in feed, fish, shrimp, tissue, egg, honey, milk, serum and urine sample.
2. Principle
This kit uses a direct competitive ELISA method in microplate coated with FB1 coupled antigen, adding FB1 standard or samples, free FB1 and pore strips Pre-coated FB1 conjugated antigen compete against FB1 antibody enzyme marker color with TMB substrate, stop solution added after the color from blue to yellow, with a microplate reader detection wavelength at 450nm, the sample absorbance value and FB1 content is inversely proportional to the sample through the standard curve FB1 content.
3. Kit composed
( 1) Pre-coated FB1 coupled antigen removable microtiter plates: 1 block ( 12 wells × 8 strips)
(2) FB1 Standard: 6 bottles ( 1ml / vial), contents were: 0 ng/ml ,10ng/ml,30 ng/ml,90ng/ml,270ng/ml ,810ng/ml.
Anti- FB1 antibody conjugate: 1 bottle (6ml)
(4) Color liquid A: 1 bottle (6ml)
(5) Color liquid B: 1 bottle (6ml)
(6) Stop Solution: 1 bottle (6ml), 2M sulfuric acid.
(7) Sample Diluent: 1 bottle ( 10 × , 6ml), used for sample dilution.
(8) washing liquid concentrate: 1 bottle (20 × , 20ml), for the washer
(9) A description
Scan Wechat Qrcode
Scan Whatsapp Qrcode