Summary:

 

Purpose

Our Quick Step Rat Caspase 8 (Casp-8)ELISA Kit is to assay Casp-8 levels in Rat serum, plasma, culture media or any biological fluid.

 

Principle

This ELISA kit uses Sandwich-ELISA as the method. The Microelisa stripplate provided in this kit has been pre-coated with an antibody specific to Casp-8. Standards or samples are added to the appropriate Microelisa stripplate wells and combined to the specific antibody. Then a Horseradish Peroxidase (HRP)-conjugated antibody specific for Casp-8 is added to each Microelisa stripplate well and incubated. Free components are washed away. The TMB substrate solution is added to each well. Only those wells that contain Casp-8 and HRP conjugated Casp-8 antibody will appear blue in color and then turn yellow after the addition of the stop solution. The optical density (OD) is measured spectrophotomeCasp-8ally at a wavelength of 450 nm. The OD value is proportional to the concentration of Casp-8. You can calculate the concentration of Casp-8 in the samples by comparing the OD of the samples to the standard curve.

Typical Data

The standard curve of QS1205Ra is provided for demonstration only. A standard curve should be generated for each set of samples assayed. 

Standard	Concentration	OD Value	Average OD Value
Blank Well	0pg/ml	0.052	0.0460
		0.040
S1	3600pg/ml	1.912	1.9075
		1.903
S2	2400pg/ml	1.388	1.3840
		1.380
S3	1200pg/ml	0.708	0.7100
		0.712
S4	600pg/ml	0.403	0.4000
		0.397
S5	300pg/ml	0.189	0.1960
		0.203

Cartpieces

• 
• 1

  1Delete

• 
• 8

  8Delete

• 
• Q

  QDelete

• 
• Q

  QDelete

• 
• +

  +Delete

• 
• 7

  7Delete

• 
• 2

  2Delete

• Delete

• 
• 2

  2Delete

• 
• 1

  1Delete

• 
• $

  $Delete

• 
• 0

  0Delete

• 
• 4

  4Delete

• 
• $

  $Delete

• 
• 0

  0Delete

• 
• 0

  0Delete

• 
• 1

  1Delete

• 
• Q

  QDelete

• Delete

• 
• <

  <Delete

• 
• i

  iDelete

• 
• i

  iDelete

• Delete

• 
• 1

  1Delete

• Delete

• 
• 1

  1Delete

• 
• 1

  1Delete

• 
• 0

  0Delete

• Delete

• 
• 2

  2Delete

• 
• 1

  1Delete

• 
• 0

  0Delete

• 
• 0

  0Delete

• 
• 0

  0Delete

• 
• 0

  0Delete

• 
• 0

  0Delete

• 
• 0

  0Delete

• 
• 1

  1Delete

• 
• 1

  1Delete

• Delete

• 
• -

  -Delete

• 
• -

  -Delete

• 
• 0

  0Delete

• Delete

• Delete

• 
• 0

  0Delete

• 
• 0

  0Delete

• 
• 0

  0Delete

• 
• f

  fDelete

• Delete

• Delete

• 
• 0

  0Delete

• 
• 0

  0Delete

• Delete

• Delete

• Delete

• Delete

• Delete

• Delete

• Delete

• 
• S

  SDelete

• Delete

• Delete

• 
• 4

  4Delete

• 
• $

  $Delete

• Delete

• 
• 0

  0Delete

• Delete

• Delete

• Delete

• 
• b

  bDelete

• 
• Q

  QDelete

• 
• 8

  8Delete

• 
• Q

  QDelete

Totalgoods,subtotals:￥Checkout