Drug Names

Generic Name: Human Helicobacter pylori IgA (Hp-IgA)ELISA Kit

Purpose

Our Human Helicobacter pylori IgA (Hp-IgA)ELISA kit is to assay Hp-IgA levels in Human serum, plasma, culture media or any biological fluid.

Principle

This ELISA kit uses Sandwich-ELISA as the method. The Microelisa stripplate provided in this kit has been pre-coated with an antigen specific to Hp-IgA. Standards or samples are added to the appropriate Microelisa stripplate wells and combined to the specific antigen . Then a Horseradish Peroxidase (HRP)-conjugated antigen specific for Hp is added to each Microelisa stripplate well and incubated. Free components are washed away. The TMB substrate solution is added to each well. Only those wells that contain Hp-IgA and HRP conjugated Hp antigen will appear blue in color and then turn yellow after the addition of the stop solution. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of Hp-IgA. You can calculate the concentration of Hp-IgA in the samples by comparing the OD of the samples to the standard curve.

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