Product Description:

Resistant starch (RS) is that portion of the starch that cannot be digested and absorbed in the small intestine  of  healthy  human.  RS  is  partially  or  completely  fermented  in  the  colon,  producing  many short-chain  fatty  acids,  which  can  inhibit  the  hyperproliferation  of  intestinal  epithelial  cells,  reduce intestinal inflammation, and reduce the risk of colon cancer. RS has good physicochemical and functional properties and is widely used in the production and processing of various foods to improve product quality and nutritional structure.RS in the sample is prepared after α-amylase and amyloglucosidase hydrolyze non-resistant starch into glucose. RS is also hydrolyzed into glucose. Glucose oxidase catalyzes the oxidation of glucose to gluconic acid and hydrogen peroxide. Peroxidase catalyzes the oxidation of 4-aminoantipyrine bisphenol by hydrogen peroxide to form colored compounds with characteristic absorption peaks at 510 nm.

Reagents and Equipment Required but Not Provided:

Spectrophotometer/microplate reader, mortar/grinding mill, 30-50 mesh  sieve, balance, centrifuge, shaking water bath, water bath/metal bath, magnetic stirrer, magnetic stirrer bars, adjustable pipette, micro glass cuvette/96 well plate, 2mL tube, 5mL tube, 50mL tube, ice and distilled water.

 

 

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