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Drug Names
Generic Name:Human 5-Hydroxytryptamine(5-HT) ELISA Kit
Purpose
Our Human 5-Hydroxytryptamine(5-HT)ELISA kit is to assay 5-HT levels in Human serum, plasma, culture media or any biological fluid.
Principle
This ELISA kit uses Sandwich-ELISA as the method. The Microelisa stripplate provided in this kit has been pre-coated with an antibody specific to 5-HT. Standards or samples are added to the appropriate Microelisa stripplate wells and combined to the specific antibody. Then a Horseradish Peroxidase (HRP)- conjugated antibody specific for 5-HT is added to each Microelisa stripplate well and incubated. Free components are washed away. The TMB substHumane solution is added to each well. Only those wells that contain 5-HT and HRP conjugated 5-HT antibody will appear blue in color and then turn yellow after the addition of the stop solution. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentHumanion of 5-HT. You can calculate the concentHumanion of 5-HT in the samples by comparing the OD of the samples to the standard curve.
Username | Quantity | bought time |
Al*** | 1 | 2024-06-15 |
Le*** | 1 | 2022-12-16 |
Sa*** | 1 | 2022-07-31 |
Pe*** | 1 | 2022-05-30 |
Sa*** | 2 | 2021-12-11 |
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