Granule-Bound Starch Synthase (GBSS) Assay Kit

Note: Take two or three different samples for prediction before test.

Operation Equipment: Spectrophotometer

Cat No: AK0364

Size:50T/48S

Description:

Granule-Bound Starch Synthase (GBSS ，EC 2.4. 1.21) is present in the amyloid body in a bound state, catalyzing the  elongation reaction  of the  starch  chain,  and is mainly responsible  for the  synthesis  of amylose.

 

GBSS catalyzes the reaction of ADPG with  starch primer (glucan), transferring glucose molecules to starch primers, and simultaneously generating ADP. Further, the pyruvate kinase, hexokinase and glucose- 6-phosphate dehydrogenase added in the reaction system sequentially catalyze the reduction of NADP+ to NADPH, wherein the amount of NADPH is proportional to the amount of ADP produced by the previous reaction, and the NADPH is measured at 340 nm. Increase the amount to calculate GBSS activity.

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